DELAY AND QUADRIPARTITION IN SEA URCHIN EGGS INDUCED BY SHORT EXPOSURE TO 2-MERCAPTOETHANOL
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چکیده
منابع مشابه
Cytasters induced within unfertilized sea-urchin eggs.
Conditions that induce the formation of asters in unfertilized sea-urchin eggs have been investigated. Monasters were formed by treatment of eggs with acidic or basic sea-water, or procaine- or thymol-containing sea-water. A second treatment step, incubation with D2O-containing, ethanol-containing or hypertonic sea-water induced multiple cytasters. The number and size of cytasters varied accord...
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The effects of Colcemid, the deacetyl-N-methyl derivative of colchicine, on the eggs of Arbacia punctulata were investigated. Colcemid in concentrations of 2.7 x 10(-5)M or greater blocks syngamy (the fusion of the pronuclei) in these eggs. Although a tenfold decrease in concentration of Colcemid usually permits the pronuclei to fuse, the subsequent division is blocked. In the sea urchin egg, t...
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Biochemical and immunological analysis of unfertilized sea urchin eggs has revealed the presence of at least two distinct isoforms of cytoplasmic dyneins, one soluble and the other microtubule-associated. The soluble enzyme is a 20 S particle with a MgATPase activity that can be activated 5-fold by nonionic detergents. It contains heavy chain polypeptides that 1) comigrate with the dynein heavy...
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Rhodamine (rh) phalloidin was used as a probe for actin filaments in living eggs and was observed using confocal microscopy. Exogenous, rh phalloidin labeled actin filaments were injected into eggs and were observed to translocate in the cytoplasm. At fertilization, filaments accumulated at the fertilization cone. Rh phalloidin alone, injected at concentrations that did not interfere with ferti...
متن کاملCleavage of Sea-urchin Eggs in Colchicine
INTRODUCTION We have recently put forward a theory linking mitosis with cleavage, in which we suggest that cleavage is caused by an expansion of the cell cortex which starts at either pole of the cell and later moves round to the region of the furrow (Mitchison, 1952). There is evidence that this expansion is brought about by substances diffusing from the two groups of daughter chromosomes whic...
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ژورنال
عنوان ژورنال: The Biological Bulletin
سال: 1971
ISSN: 0006-3185,1939-8697
DOI: 10.2307/1540078